UV/VIS: Instruments

UV-Vis Spectroscopy is an analytical method used to measure the absorbance of ultra-violet or visible radiation through an analayte.

UV-Visible spectroscopy is a mature and well established analytical technique used extensively in many industry sectors including Environmental Analysis, Pharmaceutical Testing, Food and Beverage Production to name but a few.

The UV-Vis Spectrophotometer is the analytical instrument used for the UV-Vis spectroscopic analysis.
Spectrophotometers are available in different configurations however most can be categorized into either single beam, split beam or double beam types depending on the design of their optical system.

Such types of instrument comprise the following components in their constructions:
Light Source
Monochromator
Cell Compartment
Detector
Signal Processing System
Split Beam Spectroscopy

The Split beam approach to UV-Vis Spectroscopy uses a single beam of light separated into Sample and Reference beams by means of a beam splitter using a separate detector for each.
Split beam instruments have a reference detector housed inside the instrument optics offering the advantage of optical stability as in double beam spectroscopy whilst using the single beam measurement technique.

The double beam approach to UV-Vis spectroscopy requires two beams of light, both having the same intensity to measure the Absorbance through sample and reference positions simultaneously.
The Sample position is used for measurement of the analyte, whereas the reference position is used for the correction against a blank solution or sample matrix.
A clear advantage of the double beam optical system is the improvement in measurement stability and drift precision as a result of having a real-time feedback of both the reference and sample signals.

We offer an extensive range of UV and Visible Spectroscopy instrumentation guaranteed to meet the needs of your application.